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PCR cloning protocols / Edited by Bing-Yuan Chen and Harry W. Janes

Colaborador(es): Idioma: Inglés Series Methods in Molecular BiologyTotowa, New Jersey : Humana Press , 2010Edición: 2nd editionDescripción: xiv, 439 páginasTipo de contenido:
  • text
Tipo de medio:
  • unmediated
Tipo de soporte:
  • volume
ISBN:
  • 9781592591770
Tema(s): Clasificación CDD:
  • 572.86  P348c 2010
Contenidos:
1. Polymerase chain reaction: basic principIes and routine practice.
2. Computer programs for PCR primer design and analysis.
3. Single-step PCR Optimization. Using touchdown and stepdown PCR programming.
4. XL PCR amplification of long targets from genomic DNA.
5. Coupled one-step reverse transcription and polymerase chain reaction procedure for cloning large cDNA fragments.
6. Long distance reverse-transcription PCR.
7. Increasing PCR sensitivity for amplification from paraffin-embedded tissues.
8. GC-rich template amplification by inverse PCA: DNA polymerase and solvent effects.
9. PCR procedure for the isolation of trinucleotide repeats.
10. Methylation-Specific PCR.
11. Direct cloning of full-Length cell differentially expressed genes by multiple rounds of subtractive hybridization.
12. Cloning PCR products: an overview.
13. Using T4 DNA polymerase to generate clonable PCR products.
14. Enzyme-free Cloning of PCR products and fusion protein expression.
15. Directional restriction site-free insertion of PCR products into vectors.
16. Autosticky PCR: directional cloning of PCR products with preformed 5' overhangs.
17. A Rapid and simple procedure for direct cloning of PCR products into baculoviruses.
18. PCR approaches to DNA mutagenesis and recombination: an overview.
19. In-frame cloning of synthetic genes using PCR inserts.
20. Megaprimer PCR.
21. PCR-mediated recombination: a general method applied to construct chimeric infectious molecular clones.
22. PCR method for generating multiple mutations at adjacent sites.
23. A fast polymerase chain reaction-mediated strategy for introducing repeat expansions into CAG-repeat containing genes.
24. PCR screening in signature-tagged mutagenesis of essential genes.
25. Staggered extension process (StEP) in vitro recombination.
26. Random mutagenesis by whole-plasmid PCR amplification.
27. PCH-based strategies to clone unknown DNA regions from known foreign integrants: an overview.
28. Long distance vectorette PCR (LDV PCR).
29. Nonspecific, nested suppression PCR method for isolation of unknown flanking DNA ("Cold-start method").
30. Inverse PCR: cDNA Cloning.
31. Inverse PCR: genomic cDNA Cloning.
32. Gene cloning and expression profiling by rapid amplification of gene inserts with universal vector primers.
33. The isolation of DNA sequences flanking Tn5 transposon insertions by Inverse PCR.
34. Rapid amplification of genomic DNA sequences tagged by insertional mutagenesis.
35. Isolation of large terminal sequences of BAC inserts based on double-restriction-enzyme digestion followed by anchored PCR.
36. A "Step Oawn" PCR-based technique far walking inta and the subsequent oirect sequence analysis af flanking genamic ONA.
37. Use af PCR in library screening: an overview.
38. Claning af hamalagaus genes by gene-capture PCR.
39. Rapid and nanradiaactive dcreening af recambinant libraries by PCR.
40. Rapid cDNA claning by PCR screening (RC-PCR).
41. Generatian and PCR screening af bacteriaphage a sublibraries enriched far rare Clanes (the "Sublibrary Method").
42. PCR-based screening far bacterial artificial chramasame libraries.
43. A 384 - well microtiter-plate-based template preparation and sequencing method.
44. A Microtiter-plate-based high throughput PCR product.
Existencias
Tipo de ítem Biblioteca actual Colección Signatura topográfica Copia número Estado Fecha de vencimiento Código de barras
Libros Biblioteca Central Estantería General 572.86 P348c 2010 (Navegar estantería(Abre debajo)) c.1 Disponible 35605001956948
Libros Biblioteca Central Estantería General 572.86 P348c 2010 (Navegar estantería(Abre debajo)) c.2 Disponible 35605001956931

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Referencias bibliográficas

1. Polymerase chain reaction: basic principIes and routine practice.

2. Computer programs for PCR primer design and analysis.

3. Single-step PCR Optimization. Using touchdown and stepdown PCR programming.

4. XL PCR amplification of long targets from genomic DNA.

5. Coupled one-step reverse transcription and polymerase chain reaction procedure for cloning large cDNA fragments.

6. Long distance reverse-transcription PCR.

7. Increasing PCR sensitivity for amplification from paraffin-embedded tissues.

8. GC-rich template amplification by inverse PCA: DNA polymerase and solvent effects.

9. PCR procedure for the isolation of trinucleotide repeats.

10. Methylation-Specific PCR.

11. Direct cloning of full-Length cell differentially expressed genes by multiple rounds of subtractive hybridization.

12. Cloning PCR products: an overview.

13. Using T4 DNA polymerase to generate clonable PCR products.

14. Enzyme-free Cloning of PCR products and fusion protein expression.

15. Directional restriction site-free insertion of PCR products into vectors.

16. Autosticky PCR: directional cloning of PCR products with preformed 5' overhangs.

17. A Rapid and simple procedure for direct cloning of PCR products into baculoviruses.

18. PCR approaches to DNA mutagenesis and recombination: an overview.

19. In-frame cloning of synthetic genes using PCR inserts.

20. Megaprimer PCR.

21. PCR-mediated recombination: a general method applied to construct chimeric infectious molecular clones.

22. PCR method for generating multiple mutations at adjacent sites.

23. A fast polymerase chain reaction-mediated strategy for introducing repeat expansions into CAG-repeat containing genes.

24. PCR screening in signature-tagged mutagenesis of essential genes.

25. Staggered extension process (StEP) in vitro recombination.

26. Random mutagenesis by whole-plasmid PCR amplification.

27. PCH-based strategies to clone unknown DNA regions from known foreign integrants: an overview.

28. Long distance vectorette PCR (LDV PCR).

29. Nonspecific, nested suppression PCR method for isolation of unknown flanking DNA ("Cold-start method").

30. Inverse PCR: cDNA Cloning.

31. Inverse PCR: genomic cDNA Cloning.

32. Gene cloning and expression profiling by rapid amplification of gene inserts with universal vector primers.

33. The isolation of DNA sequences flanking Tn5 transposon insertions by Inverse PCR.

34. Rapid amplification of genomic DNA sequences tagged by insertional mutagenesis.

35. Isolation of large terminal sequences of BAC inserts based on double-restriction-enzyme digestion followed by anchored PCR.

36. A "Step Oawn" PCR-based technique far walking inta and the subsequent oirect sequence analysis af flanking genamic ONA.

37. Use af PCR in library screening: an overview.

38. Claning af hamalagaus genes by gene-capture PCR.

39. Rapid and nanradiaactive dcreening af recambinant libraries by PCR.

40. Rapid cDNA claning by PCR screening (RC-PCR).

41. Generatian and PCR screening af bacteriaphage a sublibraries enriched far rare Clanes (the "Sublibrary Method").

42. PCR-based screening far bacterial artificial chramasame libraries.

43. A 384 - well microtiter-plate-based template preparation and sequencing method.

44. A Microtiter-plate-based high throughput PCR product.