TY  - BOOK
AU  - Freshney,R.Ian
TI  - Culture of animal cells
SN  - 0471453293
U1  - 571.638  
PY  - 2005///
CY  - Hoboken, New Yersey : 
PB  - Wiley-Liss , 
KW  - Células cultivadas
KW  - Células
KW  - Biología
N1  - Incluye contenido, índice, glosario, a`endices, problemas resueltos y lista de figuras y láminas; Referencias bibliográficas : páginas 579-626; 1. Introduction. -- 2. Training programs. -- 3. Biology of cultured cells. -- 4. Laboratory design and layout. -- 5. Equipment. -- 6. Aseptic technique. -- 7. Safety, bioethics, and validation. -- 8. Culture vessels and substrates. -- 9. Oefined media and supplements. -- 10. Serum-free media. -- 11. Preparation and sterilization. -- 12. Primary culture. -- 13. Subculture and celllines. -- 14. Cloning and selection. -- 15. Cell separation. -- 16. Characterization. -- 17. Differentiation. -- 18. Transformation and immortalization. -- 19. Contamination. -- 20. Cryopreservation. -- 21. Quantitation. -- 22. Cytotoxicity. -- 23. Culture of specific cell types. -- 24. Culture of tumor cells. -- 25. Organotypic culture. -- 26. Scale-up. -- 27. Specialized techniques. -- 28. Problem solving. -- 29. In conclusion
UR  - http://www.bib.ufro.cl/libros/570/Contenido/46509.pdf
UR  - http://www.bib.ufro.cl/libros/570/Cubierta/46509.jpg
ER  -