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Chilean crude extract of Ruta graveolens generates vasodilatation in rat aorta at cellular subtoxic concentrations. [Artículo de revista] / Ignacio Jofré Fernández, Patricia Navarrete Gómez, Jorge Parodi, Fernando Romero Mejía, Raúl Salvatici Salazar ; profesora guía : Patricia Navarrete Gómez.

Por: Colaborador(es): Idioma: Inglés Temuco (Chile) : Universidad de La Frontera , 2013Descripción: 29-36 páginas: cuadros, gráficosTipo de contenido:
  • text
Tipo de medio:
  • computer
Tipo de soporte:
  • online resource
Tema(s): Recursos en línea: Nota de disertación: Tesis : (Biotecnólogo).-- Universidad de La Frontera, Facultad de Ciencias Agropecuarias y Medioambiente, 2013. Resumen: In Chile elevated percentage of population have car- diovascular diseases, 70% of this populations is a hy- pertensive peoples. The Ruta graveolens (Ruta) is a medicinal plant used in different parts of the world with different therapeutics properties like derma- tologic as far as anti-helmintic properties. We ana- lyzed the vascular action measuring the tension to identify vasodilator effect of Ruta extract in norm- tense rat?s aorta incubated and measured in isolated organ bath, and evaluating the toxicity effect in CRL-1730 cell line, through enzymatic assay (MTT), confocal microscopy (propidium iodide stain) and flow cytometry (TUNEL assay), including extracellu- lar reactive oxygen species (ROS) production through luminescence assay. The results show with DE50 29 ± 0.1 ?g/mL evidenced vasodilatation, partially endo- thelium-depend. The cytotoxicity showed with DE50 304.6 ± 2 ?g/mL in enzymatic assay (MTT) while evi- denced membrane permeability in high concentra- tions (1500 ?g/mL), DNA fragmentation in absence of oxidative stress in only observed when high concen- trations of Ruta are used over the cell culture. The vasodilatation activity is executed in subtoxic concen- tration and partially endothelium-depend without permeability effect in the membrane and deteriora- tion of the cells viability suggesting a complex effect of Ruta preparation in the regulation of vascular tone
Existencias
Tipo de ítem Biblioteca actual Colección Signatura topográfica Copia número Estado Fecha de vencimiento Código de barras
Tesis y proyectos de título Biblioteca Central Estantería Tesis y trabajos de título BIO J648ch 2013 (Navegar estantería(Abre debajo)) c.1 No para préstamo 35605002306635

Artículo de revista presentado como tesis para la Facultad de Ciencias Agropecuarias y Forestales

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Tesis : (Biotecnólogo).-- Universidad de La Frontera, Facultad de Ciencias Agropecuarias y Medioambiente, 2013.

Bibliografía

In Chile elevated percentage of population have car- diovascular diseases, 70% of this populations is a hy- pertensive peoples. The Ruta graveolens (Ruta) is a medicinal plant used in different parts of the world with different therapeutics properties like derma- tologic as far as anti-helmintic properties. We ana- lyzed the vascular action measuring the tension to identify vasodilator effect of Ruta extract in norm- tense rat?s aorta incubated and measured in isolated organ bath, and evaluating the toxicity effect in CRL-1730 cell line, through enzymatic assay (MTT), confocal microscopy (propidium iodide stain) and flow cytometry (TUNEL assay), including extracellu- lar reactive oxygen species (ROS) production through luminescence assay. The results show with DE50 29 ± 0.1 ?g/mL evidenced vasodilatation, partially endo- thelium-depend. The cytotoxicity showed with DE50 304.6 ± 2 ?g/mL in enzymatic assay (MTT) while evi- denced membrane permeability in high concentra- tions (1500 ?g/mL), DNA fragmentation in absence of oxidative stress in only observed when high concen- trations of Ruta are used over the cell culture. The vasodilatation activity is executed in subtoxic concen- tration and partially endothelium-depend without permeability effect in the membrane and deteriora- tion of the cells viability suggesting a complex effect of Ruta preparation in the regulation of vascular tone