000			02076cam a22003857a 4500
001			67587
005			20220329093301.0
007			ta
008			090327d2005 xxua gr 00110 eng d
020			_a0471453293
035			_a(Sirsi) 46509
035			_a67587
037			_aCarrera : _bBiotecnología
040			_aUFRO _cCL-TeU _bspa _erda
041			_aInglés
082	0	4	_a571.638 _bF885c 2005
100	1	0	_aFreshney, R. Ian ,
245	1	0	_aCulture of animal cells / _cR. Ian Freshney.
250			_a5th edition.
264			_aHoboken, New Yersey : _bWiley-Liss , _c2005.
300			_a642 páginas : _bilustraciones (algunas a color.) ; _c29 cm.
336			_2rdaconctent _atext _btxt
337			_2rdamedia _aunmediated _bn
338			_avolume _bnc _crdacarrier
349			_aLibro
500			_aIncluye contenido, índice, glosario, a`endices, problemas resueltos y lista de figuras y láminas
504			_aReferencias bibliográficas : páginas 579-626
505			_a1. Introduction. -- 2. Training programs. -- 3. Biology of cultured cells. -- 4. Laboratory design and layout. -- 5. Equipment. -- 6. Aseptic technique. -- 7. Safety, bioethics, and validation. -- 8. Culture vessels and substrates. -- 9. Oefined media and supplements. -- 10. Serum-free media. -- 11. Preparation and sterilization. -- 12. Primary culture. -- 13. Subculture and celllines. -- 14. Cloning and selection. -- 15. Cell separation. -- 16. Characterization. -- 17. Differentiation. -- 18. Transformation and immortalization. -- 19. Contamination. -- 20. Cryopreservation. -- 21. Quantitation. -- 22. Cytotoxicity. -- 23. Culture of specific cell types. -- 24. Culture of tumor cells. -- 25. Organotypic culture. -- 26. Scale-up. -- 27. Specialized techniques. -- 28. Problem solving. -- 29. In conclusion.
650	0	0	_aCélulas cultivadas
650	0	0	_aCélulas
650	0	0	_aBiología
856	4	0	_3Contenido _uhttp://www.bib.ufro.cl/libros/570/Contenido/46509.pdf
856	4	0	_3Cubierta _uhttp://www.bib.ufro.cl/libros/570/Cubierta/46509.jpg
942			_cLIB _2ddc
999			_c67587 _d67587