| 000 | 03312cmm a2200445li 4500 | ||
|---|---|---|---|
| 001 | 77366 | ||
| 005 | 20220909155950.0 | ||
| 007 | co*bu nnnu|auu | ||
| 008 | 171024s2014 cl s m s eng u | ||
| 035 | _a(Sirsi) a42366 | ||
| 035 | _a77366 | ||
| 040 |
_aCL-TeUF _b spa _c CL-TeU _e rda |
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| 041 | 0 | _aInglés | |
| 090 | 0 | 4 |
_aBIO _bB826e 2014 |
| 100 | 1 | _aBravo Maldonado, Anita Camila | |
| 245 | 1 | 0 |
_aEffect of mitochondrial calcium uniporter blocking on human spermatozoa _b [recurso electrónico] / _cAnita Camila Bravo Maldonado, Favian Franklin Treulen Seguel, Pamela Uribe Catalán, Rodrigo Boguen Ojeda, Ricardo Felmer Dörner, Juana Villegas Moraga ; profesor guía : Ricardo Felmer Dörner. |
| 264 | 1 |
_aTemuco (Chile) : _bUniversidad de La Frontera , _c2014. |
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| 300 |
_a24 hojas : _bfiguras. |
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| 336 |
_atext _btxt _2 rdacontent |
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| 337 |
_acomputer _b c _2 rdamedia |
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| 338 |
_aonline resource _b cd _crdacarrier |
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| 349 | _aTesis | ||
| 500 | _aArtículo de revista presentado como tesis para la Facultad de Ciencias Agropecuarias y Forestales. | ||
| 500 | _aCD-ROM contiene artículo en formato PDF (598 KB) | ||
| 500 | _aIncluye figuras, tablas, anexos.. | ||
| 502 | _aTesis : (Biotecnólogo).-- Universidad de La Frontera, Facultad de Ciencias Agropecuarias y Medioambiente, 2014. | ||
| 504 | _aBibliografía | ||
| 520 | _aCalcium (Ca2+) regulates a number of essential processes in spermatozoa. Ca2+ is taken up by mitochondria via the mitochondrial calcium uniporter (mCU). Oxygen-bridged dinuclear ruthenium amine complex (Ru360) has been used to study mCU because it is a potent and specific inhibitor of this channel. In bovine spermatozoa, it has been demonstrated that mitochondrial calcium uptake inhibition adversely affects the capacitation process. It has been demonstrated in human spermatozoa that mCU blocking, through Ru360, prevents apoptosis; however, the contribution of the mCU to normal human sperm function has not been studied. Therefore, the aim of this study was to evaluate the effect of mCU blocking on human sperm function. Spermatozoa obtained from apparently healthy donors were incubated with 5 and 10 ?m Ru360 for 4 h at 37 °C. Viability was assessed using propidium iodide staining; motility was determined by computer-aided sperm analysis, adenosine triphosphate (ATP) levels using a luminescence-based method, mitochondrial membrane potential (??m) using JC-1 staining and reactive oxygen species (ROS) production using dihydroethidium dye. Our results show that mCU blocking significantly reduced total sperm motility and ATP levels without affecting sperm viability, ??m and ROS production. In conclusion, mCU contributes to the maintenance of sperm motility and ATP levels in human spermatozoa. | ||
| 650 | 0 | 0 | _aEspermatozoides |
| 650 | 0 | 4 |
_aReproducción humana _x Investigaciones |
| 650 | 0 | 0 | _aBiotecnología |
| 700 | 1 |
_aFelmer Dörner, Ricardo _eProfesor guía |
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| 700 | 1 | _aUribe Catalán, Pamela | |
| 700 | 1 | _aTreulen Seguel, Favian Franklin | |
| 700 | 1 | _aBoguen Ojeda, Rodrigo Esteban | |
| 700 | 1 | _aFelmer Dörner, Ricardo | |
| 700 | 1 | _aVillegas Moraga, Juana Valentina | |
| 710 | 2 |
_aUniversidad de La Frontera (Chile). _bFacultad de Ciencias Agropecuarias y Medioambiente |
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_cTES _2ddc |
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_c77366 _d77366 |
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